Jamestown, or Jamestown: Legend of the Lost Colony is a shooter game that can accommodate up to four players. The player assumes the role of the English explorer Sir Walter Raleigh during an alternate place in history. The era is the same as Raleigh’s time—the 17th Century British Colonial period—but instead of the Earth, the game is set in Mars. Players are tasked to investigate the disappearance of the colony called the Roanoke. As they progress, players must fight rival Spanish forces as well as Martians.
Players must shoot the enemies that are coming from the top of the screen. At the beginning of the game, players have a laser shooter. Players can use two firing types, namely standard and alt-fire. Standard shots are light-damage shots, while alt-fire brings heavy damage to the targets. When shot and destroyed, the enemies drop gold that players can collect for points. When players have amassed 2000 points, they can buy an advanced firearm called the Gunner, which also has a standard fire and an alt-fire.
Jamestown is comprised of five chapters. While chapters 1 and 2 can be played on any level of difficulty, the third chapter can only be unlocked if the previous ones are completed on Difficult or higher. The fourth and fifth levels may only be unlocked if Levels 1-3 are completed on the Legendary level of difficulty.